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Introduction info

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  • Overview

The small brown planthopper (SBPH), Laodelphax striatellusFallén (Hemiptera: Delphacidae) is a hemimetabolous insect of which body size is less than 5 mm and has diploid bisexual chromosome (2n = 28 + XX or XO). SBPH is widely distributed in Asia, Europe, and Northern Africa and mainly feeds on the grass family, Poaceae. SBPH is one of the major pests against rice plant, Oryza sativaL. in East Asia. High density of SBPH could cause severe economic damage on rice plant by directly sucking and indirectly transmitting viral pathogens, rice stripe virus and rice streaked dwarf virus, with piercing mouthparts (i.e. Stylets). Rice stripe virus can be transmitted in a circulative, persistent, propagative manner by SBPH. SBPH has wing-size dimorphism (short vs. long) depending on genetic and environmental effect. A long-winged SBPH has been reported to massively migrate from China to Korea and Japan in early summer season. Nymphs of SBPH can also overwinter in temperate regions. Furthermore, SBPH has developed high resistance to diverse classes of insecticides including organophosphates, neonicotinoids, chitin biosynthesis inhibitor, etc., in the laboratory and the field. SBPH is taxonomically close to the brown planthopper (BPH), Nilaparvata luges (Stål) (Hemiptera: Delphacidae) which is another destructive rice pest, but diverse host range, different kind of virus transmission, and cold tolerance of SBPH are quite different from those of BPH. All these features could result in SBPH management problem in East Asia, so that genome-wide study through whole-genomesequencing may provide valuable insight tobetter understand the biological system of SBPH and the interrelationship among rice plant, virus,SBPH, and environmental factors and to develop new target site for pest management.

 

  • Statistics

A draft genome of L. striatellus has been constructed by using PacBio and Illumina NGS platforms. It consists of 565Mbp in total length, 1,598 in scaffold contig number, and 806kbp in N50 size. A pseudomolecule of L. striatellus has been constructed by linkage mapping and scaffold anchoring with SNP marker after genotyping by sequencing. For publication on a draft genome and pseudomolecule of L. striatellus, more detailed analysis is in progress.

 

Table 1. Summary of de novo assembly for whole genome of Laodelphax striatellus with PacBio sequencing platform

NGS

No. of Scaffolds

Whole genome of Laodelphax striatellus

[length (bp)]

GC

(%)

Total

Min.

Max.

Avg.

N50

N

 

(PacBio + WGA)

(gap filling)

1,598

565,774,043

20,023

4,232,725

354,051

806,222

3,673,236

34.25

 

 

  • Contact information

  • Principal Investigator: Seo, Bo Yoon Ph.D (Entomological researcher, National Institute of Agricultural Sciences, Rural Development Administration, Wanju, South Korea)

  • Tel: +82-63-238-3289

  • Fax: +82-63-238-3838

  • Email: seoby@korea.kr

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